in reply to appending to a file

I'd start with error checking. (added: Fletch is more accurate than I am on the functions you'll want to check errors on, consider adding similar error checking to the functions mentioned):
open( my $in, ">>".$seq_dir."/".$fa) or die "Unable to open $seq_dir/$fa for append: $!\n";
Obviously if the file is read only or the path isn't parsed correctly, the open call will fail and you won't be able to write to the file.