It depends what you are trying to do. My preferred profiler is "look at the code and think real hard". Primitive, but gives the best bang for the buck most the time. If I have to muck about with an actual profiler, I remind myself that Perl is a Very High Level Language, and maybe it is time to code the performance sensitive bits in C.

But to answer your question, DProf and Benchmark are where you want to go once you determine you need to profile.