use Fcntl qw(SEEK_SET);

my $input = '...';

my $start  = 1_490_117;
my $end    = 1_492_312;
my $length = $end - $start +1;

open my $fh, '<', $input or die "Can't open $input: $!";
sysseek($fh, $start, SEEK_SET) or die "Can't seek to $start in $input: $!";
my $sequence;
my $read = sysread $fh, $sequence, $length;
die "Failed to read $length bytes from $input, got $read"
  if $length != $read;