I'd say that normally (99% of the time) I write the subject line first before writing the body. I consider email the equivalent of writing a memo or (I'm about to date myself) a paper letter. Usually when I write a letter to someone (like a vendor or client) I have a subject line right up there with the address portion of the letter such as:

                                         Shark River Technical Solutions
                                         123 Mystreet St.
                                         City, State   00000-0000
                                         (555) 555-1212
                                          September 10, 2012

Client Name
321 TheirStreet St.
City, State    11111-1234

Subject:  Account 123456-07 in arrears

And in fact I have my own templates for LibreOffice already built for both personal letters and business letters that plug the information in for me (since I'm lazy.)

I have, however, been known to go back and change the subject line based on the content of an email if the direction of that email changed since I first thought of writing it.

I've also been known to change a subject line for a reply to an email. For instance someone has written me an email with the subject line "Hi There!" I'll change the subject line to something like "RE: Dinner plans for Saturday (was RE: Hi There!)" so that looking at the thread later on makes sense to me.

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