No you don't fill @F. The -a switch does it for you. see perldoc perlrun
The code above is complete script. Just run it as shown in usage.
Now explanations:
#!/usr/bin/perl -lan
# Usage: thisscript.pl genes.txt
# %seen is a hash where we store keys composed from seen rows
# @F is an array of 2 elements $F[0] is a first column of your file an
+d $F[1] is the second one (see [doc://perlrun] -a switch
# So the key for row is composed by concatenation of sorted columns
if ( $seen{ join ' ', sort @F }++ ) {
# %uniq hash will keep seen columns first and second
$uniq{$F[0]}++;
$uniq{$F[1]}++;
}
# the code above is loops over each row of file because of -n switch s
+ee [doc://perlrun]
END { # this block run just before exit
print for keys %uniq;
}
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