Beefy Boxes and Bandwidth Generously Provided by pair Networks
Your skill will accomplish
what the force of many cannot
 
PerlMonks  

comment on

( [id://3333]=superdoc: print w/replies, xml ) Need Help??

I am really new to perl and am taking a course on it. I wrote the following program for an assignment and am getting the incorrect output. I'm getting over a million lines while the expected output is closer to 250,000. The last 12 nts need to be unique to the genome. I have a feeling it's due to my regex. Any advice would be greatly appreciated. Thankyou.

#!/usr/bin/perl use strict; use warnings; my %windowSeqScore = (); my $input_file = '/scratch/Drosophila/dmel-all-chromosome-r6.02.fasta' +; my $sequenceRef = loadSequence($input_file); my $output_file = 'unique12KmersEndingGG.fasta'; open (KMERS,">", $output_file) or die $!; my $windowSize = 21; my $stepSize = 1; for ( my $windowStart = 0 ; $windowStart <= ( length ( $$sequenceRef ) + - $windowSize ); $windowStart += $stepSize ) { my $windowSeq = substr ( $$sequenceRef, $windowStart, $windowS +ize); if ($windowSeq =~ /([ATCG]{10}GG$)/) { $windowSeqScore{$windowSeq}++; } } my $count = 0; for (keys %windowSeqScore){ $count ++; if ($windowSeqScore{$_} == 1 ) { print KMERS ">crispr_$count", "\n", $_, "\n"; } } sub loadSequence { my ($sequenceFile) = @_; my $sequence = ""; unless ( open( FASTA, "<", $sequenceFile ) ) { die $!; } while (<FASTA>){ my $line = $_; chomp ($line); if ($line !~ /^>/ ) { $sequence .= $line; } } return \$sequence; }

This is some of the output I'm getting

>crispr_1 ACAACAATAATGCGACGATGG >crispr_2 TCCGAAGTCTGCCACTTTAGG >crispr_3 TGATTCCCGATGCAGTGGGGG >crispr_4 GTGGGACGACTGGACAAGTGG >crispr_5 GCCGAAGGAACAACACACAGG >crispr_7 CAAAGTCACTGTCTACGCAGG >crispr_8 ATCATTTGCTACCAGAAATGG >crispr_9 ATCCTGCCTGGCAGCCGGAGG >crispr_10 CCCTTGATCATGATAAATGGG >crispr_11 AACAACTAACTCATTTTGTGG >crispr_12 TTCCCAGCGGGGAAAAAATGG >crispr_13 TCAAGAAAGATTTCCAAAAGG >crispr_14 CCATGCGAGAAATCGCGCAGG >crispr_15 GCTGCTCAAACTGGAACTTGG

this is some of the expected output I should be getting

>crispr_1 TTTAGACTCCCCTTGTACAGG >crispr_2 TCTTCAGTCTCCAGTCTCCGG >crispr_3 TTGCGTTGCGGAGCATACTGG >crispr_4 TGCCACCAGTGGTTCCAAGGG >crispr_5 TTATGTTTGTACGAGGGGGGG >crispr_6 TCTCTTTGGTTTACGGATGGG >crispr_7 TTGGCAAGGAGACGGTCCTGG >crispr_8 TGAATTAAAGCTTGCGCGAGG >crispr_9 GGAAGAGGCATCAACGAGGGG >crispr_10 TGCAGCGGCCTAACAAGGCGG >crispr_11 CTGCCCGATCCTAACTCCAGG >crispr_12 ATATATGTTTGACCGTCGGGG >crispr_13 GGAAACAAAAGCCTATGCGGG >crispr_14 TGCGATCAGGTGTATCCGAGG

In reply to unique sequences by Anonymous Monk

Title:
Use:  <p> text here (a paragraph) </p>
and:  <code> code here </code>
to format your post; it's "PerlMonks-approved HTML":



  • Are you posting in the right place? Check out Where do I post X? to know for sure.
  • Posts may use any of the Perl Monks Approved HTML tags. Currently these include the following:
    <code> <a> <b> <big> <blockquote> <br /> <dd> <dl> <dt> <em> <font> <h1> <h2> <h3> <h4> <h5> <h6> <hr /> <i> <li> <nbsp> <ol> <p> <small> <strike> <strong> <sub> <sup> <table> <td> <th> <tr> <tt> <u> <ul>
  • Snippets of code should be wrapped in <code> tags not <pre> tags. In fact, <pre> tags should generally be avoided. If they must be used, extreme care should be taken to ensure that their contents do not have long lines (<70 chars), in order to prevent horizontal scrolling (and possible janitor intervention).
  • Want more info? How to link or How to display code and escape characters are good places to start.
Log In?
Username:
Password:

What's my password?
Create A New User
Domain Nodelet?
Chatterbox?
and the web crawler heard nothing...

How do I use this?Last hourOther CB clients
Other Users?
Others having a coffee break in the Monastery: (2)
As of 2024-04-25 23:00 GMT
Sections?
Information?
Find Nodes?
Leftovers?
    Voting Booth?

    No recent polls found