Perhaps the following will be helpful:
use strict;
use warnings;
use autodie;
my ( %ids, $id );
open my $idFH, '<', 'sample_IDs.txt';
while (<$idFH>) {
$ids{$1} = $2 if /(.+)\s+(.+)/;
}
close $idFH;
open my $sampleFH, '<', 'sample_reads.fasta';
while (<$sampleFH>) {
s/\n/"_weight=$ids{$id}\n"/e
if ($id) = /^(>\S+)/ and exists $ids{$id};
print;
}
close $sampleFH;
This uses autodie to handle file-opening exceptions. Note, also, the use of lexical (my) file handles instead of barewords.
A regex captures the ID/val pairs from the IDs file, using those to build a hash. When the sample file is processed, the ID's captured. If that ID exists as a key in the hash, the \n is replaced by the desired string. All lines are printed.
Partial output on your datasets:
...
>comp10002_c0_seq3 len=99 path=[2446:0-34 1163:35-98]
TTTTTTGTGATATATTAAATAATATATAAAAATACTATGGCAGGAAGTTTAAATAAAGTC
TTATTAATAGGCCGTTTAGGCGCAGACCCAGATATAAAA
>comp10003_c0_seq1 len=166 path=[748:0-22 1004:23-46 2527:47-165]_weig
+ht=41
AAGTAGCCTATGCGCTACAGTAAGAAAGACAGGTGAAAAAATGGAAGTAAAACAATTAGA
TGACTACTTTGGATATACAGAAAAGGGCAGTTCCTTAGAGGGGGAATTACGAGCAGGACT
AACGACATTCTTGACAATGGCGTACATTCTGTTTGTGAACCCAGAC
>comp10004_c0_seq1 len=143 path=[2167:0-44 2322:45-68 2508:69-142]_wei
+ght=25
AATCTTTAATTTAAACTTAAAAAAAATTAACTTTTGAAAGGAATTAAAATGGAAAAAGAA
ATGTTAGTAGTAGCTAAATTAAAAGAAGGTACATTTGAAAAATTTATGGGTTTCATGCAA
TCGCCTGAAGGTTTAGCAGAAAG
>comp10005_c0_seq1 len=135 path=[2666:0-71 4268:72-134]_weight=96
AATATTACCAGAAGTTACAGGTGATGTGACTTATTTACATTGCTTCGGTGAGTGTTCAGG
TGATGGTACAGGTGAATGCCCAAGTGGCGCTGTAACATGGATGCTTACAATGACTGTAAA
TACTGCTAATATCAC
...