use 5.010; my $str1 = 'MALSSTAATTSSKLKLSNPPSLSHTFTASASASVSNSTSFR'; my $a_count = $str1 =~ tr/A/A/; # 6 #### #!/usr/bin/env perl use 5.010; for (<>) { if (/^>/) { # Header } elsif (/^[A-Z]+$/) { # Protein my $a = tr/A/A/; say "A: $a, length: " . length; } } #### open my $fh, '<', $filename or die "Couldn't open $filename: $!"; for (<$fh>) {