#!/usr/bin/perl use warnings; use strict; my %proteins; while (my $line = ) { chomp $line; my ($pro, $org, $value) = split '\s+, $line; # \s+ : one or more whitespace chars } __DATA__ protein1 organism1 0.843534 protein2 organism2 2.45 protein3 organism3 9.5322 protein4 organism4 0.3475474 protein1 organism6 9.4534 protein2 organism7 0.43534 protein2 organism8 1.2434 protein3 organism9 0.000003 protein3 orgnanism10 1.23325